In-silico prediction of dual function of DksA like hypothetical protein in V. cholerae O395 genome

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• 作者：Avirup Dutta¹ ; Atul Katarkar¹ ; ² ; Keya Chaudhuri ; ^{keya.chaudhuri@gmail.com} ; ^{kchaudhuri@iicb.res.in} ; ^{profkeyachaudhuri@gmail.com}
• 关键词：V. cholerae O395 ; Homology modeling ; Stringent response ; DksA like protein ; (p)ppGpp
• 刊名：Microbiological Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：195
• 期：Complete
• 页码：60-70
• 全文大小：3395 K
• 卷排序：195

文摘

Cholera, an acute infection of the small intestine, is caused by Vibrio cholerae. The present study identified a hypothetical protein in V. cholerae O395, which was predicted to be acquired through horizontal gene transfer the origin of which was found to be from a phage. Its expression was further confirmed by RT-PCR. Homology based 3D model of the hypothetical protein indicated DksA like homologue. Protein binding site of 3D-model revealed a deep cleft which may influence the dimer formation and interaction with ds-DNA molecule. Also, canonical function of direct interaction with RNA polymerase (RNAP) holoenzyme in complex with ppGpp suggests its dual role in the pathogenesis of cholera.