Production of all trans-beta-carotene by using impinging flow of supercritical carbon dioxide anti-solvent pulverization

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• 作者：Yi-Chen Shen^a ; Lee-Muei Chng^b ; Yuan-Chuen Wang^c ; Chwen-Jen Shieh^d ; Kuo-Li Lin^a ; Shih-Lan Hsu^e ; Hong-Nong Chou^f ; Chieh-Ming J. Chang^a ; ^{cmchang@dragon.nchu.edu.tw}
• 关键词：Dunaliella salina ; Trans-beta-carotene ; Column elution chromatography ; Supercritical anti-solvent precipitation
• 刊名：Journal of Chromatography A
• 出版年：2012
• 出版时间：28 December, 2012
• 年：2012
• 卷：1270
• 期：Complete
• 页码：1-8
• 全文大小：1239 K

文摘

This work investigated column elution chromatography coupled with supercritical anti-solvent precipitation to produce carotenoid rich microsized particulates from microalgal Dunaliella salina species. The extract contained carotenoids ranging from 61.3 mg/g_salina to 72.5 mg/g_salina using ultrasonic stirred ethyl ether or tetrahydrofuran (THF) extraction. When 10 L of ethyl alcohol was employed to elute the THF extract, purity of trans-¦Â-carotene is 823.6 mg/g with a recovery of 86.2 % . It was found that the supercritical anti-solvent of THF solution at 160 bar and 318 K produced powdered particulates with a purity of carotenoids above 90 % . Subsequently, a central composite response surface design method was used to design supercritical anti-solvent precipitation of carotenoid-rich THF solution. This was accomplished by increasing the pressure from 140 bar to 180 bar and the time from 40 min to 60 min at a feed flow rate of 0.2 mL/min. A CO₂ flow rate of 15 L/min and a temperature of 318 K were also used to determine the effects on purity and recovery of trans-¦Â-carotene. The combined process produced micronized precipitates with a mean particle size ranging from 3.5 ¦Ìm to 19 ¦Ìm and the purity of trans-¦Â-carotene attained was 926.8 mg/g with a recovery of 54 % .