Low density supercritical fluids precipitation of 9-cis and all trans-¦Â-carotenes enriched particulates from Dunaliella salina

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• 作者：Jian-Ren Chen ; Jia-Jiuan Wu ; Justin Chun-Te Lin ; Yuan-Chuen Wang ; Chiu-Chung Young ; Chwen-Jen Shieh ; Shih-Lan Hsu ; Cheih-Ming J. Chang
• 关键词：9-cis-¦Â-Carotene ; trans-¦Â-carotene ; Supercritical anti-solvent Pulverization ; Elution chromatography ; Microalgae
• 刊名：Journal of Chromatography A
• 出版年：2013
• 出版时间：19 July, 2013
• 年：2013
• 卷：1299
• 期：Complete
• 页码：1-9
• 全文大小：3178 K

文摘

In this study, supercritical anti-solvent (SAS) pulverization coupled with reverse phase elution chromatography was employed to isolate 9-cis and trans-¦Â-carotenes from Dunaliella salina. Total concentration of 9-cis (134.7 mg/g) and trans-¦Â-carotene (204.2 mg/g) was increased from 338.9 mg/g of the ultrasonic extract to 859.7 mg/g (338.9 for 9-cis and 520.8 for trans) of the elution fraction. The SAS pulverization of the collected fraction further produced submicron-sized particulates containing 932.1 mg/g (355.6 for 9-cis and 576.5 for trans) of total ¦Â-carotenes with a recovery of 86.3 % (83.9 % for cis and 87.8 % for trans). Effects of two SAS operational conditions on the purity, recovery of total ¦Â-carotenes, mean size and morphology of the precipitates were obtained from an experimentally designed method. Generation of micronized particulates enriched with 9-cis and trans-¦Â-carotenes by low-density SAS was proved to be feasible and environmental benign.