Mechanism of surface charge triggered intestinal epithelial tight junction opening upon chitosan nanoparticles for insulin oral delivery

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• 作者：Juan Wang^a ; ^b ; ¹ ; Ming Kong^a ; ¹ ; Zhenjin Zhou^c ; Dong Yan^a ; Xiaoping Yu^a ; Xiaojie Cheng^a ; Chao Feng^a ; Ya Liu^a ; ^{yaliu@ouc.edu.cn} ; Xiguang Chen^a ; ^{xgchen@ouc.edu.cn}
• 关键词：Surface charge ; CMCS/CS nanoparticles ; Insulin oral ; Tight junction ; Claudin-4
• 刊名：Carbohydrate Polymers
• 出版年：2017
• 出版时间：10 February 2017
• 年：2017
• 卷：157
• 期：Complete
• 页码：596-602
• 全文大小：2257 K
• 卷排序：157

文摘

Insulin blood level in negative nanoparticles was higher than positive ones. Tight junctions almost disappeared in negative nanoparticles group. Opposite charged insulin:CMCS/CS-NGs had same effect on claudin-4 protein expression. A significant down-regulation of phosphorylated claudin-4 in negative nanoparticles group. Negative nanoparticles triggered stronger paracellular permeability than positive ones.