PCNA dependent cellular activities tolerate dramatic perturbations in PCNA client interactions

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• 作者：Rosemary H.C. Wilson^a ; Antonio J. Biasutto^b ; Lihao Wang^a ; Roman Fischer^c ; Emma L. Baple^d ; Andrew H. Crosby^d ; Erika J. Mancini^b ; ^e ; Catherine M. Green^a ; ^{catherine.green@well.ox.ac.uk}
• 关键词：PCNA ; PCNA-associated repair disorder (PARD) ; DNA replication ; DNA repair
• 刊名：DNA Repair
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：50
• 期：Complete
• 页码：22-35
• 全文大小：3996 K
• 卷排序：50

文摘

We assess the cellular effects of the mutation that causes PARD (PCNAS228I). Cells from affected individuals are sensitive to T2AA and T3. PCNAS228I impairs interactions between PCNA and Cdt1, DNMT1, PolD3 and PolD4. The PIP-box of p21 retains binding to PCNAS228I. PCNA-dependent degradation and the cell cycle are only subtly altered by PCNAS228I.