Bacillus subtilis cells immobilised in PVA-cryogels

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• 作者：Szczesna-Antczak ; M. ; Galas ; E.
• 关键词：Immobilisation ; PVA-cryogels ; B. subtilis ; Proteinases ; Image analysis ; ASM ; textural coefficient of an angular second moment ; CFU ; colony forming units ; DH ; degree of hydrolysis ; DP ; degree of polymerization ; PVA ; poly(vinyl alcohol)
• 刊名：Biomolecular Engineering
• 出版年：2001
• 出版时间：January, 2001
• 年：2001
• 卷：17
• 期：2
• 页码：55-63
• 全文大小：977 K

文摘

Bacillus subtilis viable cells were immobilised in PVA-cryogel beads using the ‘freezing-thawing’ method in a two-phase (water–oil) system. Conditions providing both high thermal and mechanical stability and suitable porosity of the carrier were optimised. For monitoring and analysis of changes inside the biocatalyst beads, and for determination of diffusive properties of the carrier, an image analysis was applied. It was revealed that bacterial spores, sodium alginate and bacterial cellulose accelerated hardening of the cryogels and modified their porosity. Proteins (haemoglobin, azoalbumin, azocasein) penetrated beads of the cryogel.