Competitive binding of (−)-epigallocatechin-3-gallate and 5-fluorouracil to human serum albumin: A fluorescence and circular dichroism study

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• 作者：Lixia Yuan^a ; Min Liu^a ; ^b ; ^{panpanliumin@163.com} ; Guiqin Liu^b ; Dacheng Li^b ; Zhengping Wang^b ; Bingquan Wang^b ; Jun Han^b ; Min Zhang^c ; ^{zhangminliaoda@163.com}
• 关键词：(&minus ; )-Epigallocatechin-3-gallate (EGCG) ; 5-Fluorouracil (FU) ; Human serum albumin (HSA) ; Fluorescence quenching ; Circular dichroism (CD)
• 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：173
• 期：Complete
• 页码：584-592
• 全文大小：1004 K
• 卷排序：173

文摘

The interactions of HSA with individual or combined EGCG and FU were investigated. Site identification experiments showed that both EGCG and FU mainly bind to subdomain IIA. A competition binding model for the ternary system based on complex was proposed. The secondary structure of HSA was altered due to the binding of EGCG and FU. Competitive binding of EGCG and FU to HSA may enhance anticancer efficacy.