Establishing estrogen-responsive mouse mammary organoids from single Lgr5⁺ cells

详细信息    查看全文

• 作者：Lixing Zhang^a ; ^b ; ^c ; Mohammad Adileh^b ; Maria Laura Martin^b ; Stefan Klingler^b ; Julie White^d ; Xiaojing Ma^a ; ^e ; Louise R. Howe^c ; Anthony M.C. Brown^c ; ^e ; ¹ ; ^{amcbrown@med.cornell.edu} ; Richard Kolesnick^b ; ¹ ; ^{r-kolesnick@ski-mskcc.org}
• 关键词：Lgr5+ ; Stem cells ; Mammary gland ; Organoid
• 刊名：Cellular Signalling
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：29
• 期：Complete
• 页码：41-51
• 全文大小：2855 K
• 卷排序：29

文摘

Evidence as to whether Lgr5+ mammary cells are stem cells is inconclusive. Here we grow sustainable organoids from single murine Lgr5+ mammary cells. Organoids derived from single Lgr5+ cells display all major ductal cell types. Lgr5-derived organoids self-organize into hormone-responsive duct-like structures. We propose these structures represent “mini-breast” organoids.