A one-step duplex rRT-PCR assay for the simultaneous detection of duck hepatitis A virus genotypes 1 and 3

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A one-step duplex rRT-PCR assay for the simultaneous detection of duck hepatitis A virus genotypes 1 and 3

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作者：Qin Hu^a ; ^b ; ^c ; ^{15283521606@163.com" class="auth_mail" title="E-mail the corresponding author} ; Dekang Zhu^b ; ^c ; ¹ ; ^{zdk24@163.com" class="auth_mail" title="E-mail the corresponding author} ; Guangpeng Ma^d ; ¹ ; ^{maguangpeng@163.com" class="auth_mail" title="E-mail the corresponding author} ; Anchun Cheng^a ; ^b ; ^c ; ^{chenganchun@vip.163.com" class="auth_mail" title="E-mail the corresponding author} ; Mingshu Wang^a ; ^b ; ^c ; ^{mshwang@163.com" class="auth_mail" title="E-mail the corresponding author} ; Shun Chen^a ; ^b ; ^c ; ^{sophia_cs@163.com" class="auth_mail" title="E-mail the corresponding author} ; Renyong Jia^a ; ^b ; ^c ; ^{cqrc_jry@163.com" class="auth_mail" title="E-mail the corresponding author} ; Mafeng Liu^a ; ^b ; ^c ; ^{liumafengra@163.com" class="auth_mail" title="E-mail the corresponding author} ; Kunfeng Sun^a ; ^b ; ^c ; ^{sunkunfeng1981@163.com" class="auth_mail" title="E-mail the corresponding author} ; Qiao Yang^a ; ^b ; ^c ; ^{yangqiao721521@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Ying Wu^a ; ^b ; ^c ; ^{yingzi_no1@126.com" class="auth_mail" title="E-mail the corresponding author} ; Xiaoyue Chen^b ; ^c ; ^{chenxy_24@sina.cn" class="auth_mail" title="E-mail the corresponding author}
关键词：Duck hepatitis A virus ; DHAV-1 ; DHAV-3 ; Duplex rRT-PCR
刊名：Journal of Virological Methods
出版年：2016
出版时间：October 2016
年：2016
卷：236
期：Complete
页码：207-214
全文大小：1346 K

文摘

class="label">•: The method was sensitive, specific and time-saving that can be used as a reliable and effective tool for the diagnosis, detection and molecular epidemiology investigation of DHAV-1, DHAV-3 and their mixed infection.
class="label">•: The method of one-step duplex rRT-PCR can simultaneously detect DHAV-1 and DHAV-3 in the same tube.
class="label">•: The method can help provide timely and effective prevention and control of breeding reservoirs for DHAV.