Production of recombinant dengue non-structural 1 (NS1) proteins from clinical virus isolates

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作者：Benediktus Yohan^a ; Puspa Wardhani^b ; Aryati^b ; Hidayat Trimarsanto^a ; ^c ; R. Tedjo Sasmono^a ; ^{sasmono@eijkman.go.id" class="auth_mail" title="E-mail the corresponding author}
关键词：Dengue ; NS1 ; Recombinant protein ; Diagnostic
刊名：Protein Expression and Purification
出版年：2017
出版时间：January 2017
年：2017
卷：129
期：Complete
页码：53-59
全文大小：860 K

文摘

class="label">•: We expressed dengue NS1 proteins from Indonesia DENV clinical isolates.
class="label">•: NS1 proteins were expressed using a site-specific recombination cloning method.
class="label">•: The method can be used for producing NS1 using virus from other geographical regions with minimal modification.
class="label">•: The purified NS1 proteins were antigenically active.
class="label">•: The NS1 proteins produced can induced antibody response in mice.