High-Content Quantification of Single-Cell Immune Dynamics

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High-Content Quantification of Single-Cell Immune Dynamics

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作者：Michael Junkin¹ ; Alicia J. Kaestli¹ ; Zhang Cheng² ; Christian Jordi¹ ; Cem Albayrak¹ ; Alexander Hoffmann² ; Savaş Tay¹ ; ³ ; ⁴ ; ^{tays@uchicago.edu" class="auth_mail" title="E-mail the corresponding author}
关键词：single-cell analysis ; dynamics ; cytokine ; NF-κB ; microfluidic ; input
刊名：Cell Reports
出版年：2016
出版时间：12 April 2016
年：2016
卷：15
期：2
页码：411-422
全文大小：4446 K

文摘

class="label">•: Dynamic stimulation of single immune cells with a versatile microfluidic device
class="label">•: Coupled longitudinal measurements of NF-κB localization and TNF secretion on the same cell
class="label">•: Single-cell harvesting, staining, and mRNA quantification on the same device
class="label">•: High-content dataset, and modeling of TRIF-based noise in TNF secretion

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