A thermotolerant Endo-1,4-β-mannanase from Trichoderma virens UKM1: Cloning, recombinant expression and characterization

网站地图 | English | 公务邮箱

About the library

Background
History
Leadership
Organization

Opening Hours
Collections
Help Via Email

Electronic Information Resources

A thermotolerant Endo-1,4-β-mannanase from Trichoderma virens UKM1: Cloning, recombinant expression and characterization

详细信息查看全文

作者：Sin Yee Chai^a ; ^{sinyeechai23@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Farah Diba Abu Bakar^b ; ^{fabyff@ukm.edu.my" class="auth_mail" title="E-mail the corresponding author} ; Nor Muhammad Mahadi^c ; ^{nor.mahadi@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Abdul Munir Abdul Murad^d ; ^{munir@ukm.edu.my" class="auth_mail" title="E-mail the corresponding author} ; ^{munir8488@gmail.com" class="auth_mail" title="E-mail the corresponding author}
关键词：Trichoderma virens ; β-mannanase ; Pichia pastoris ; Recombinant protein ; Thermotolerant
刊名：Journal of Molecular Catalysis B: Enzymatic
出版年：2016
出版时间：March 2016
年：2016
卷：125
期：Complete
页码：49-57
全文大小：2196 K

文摘

class="label">•: An endo-β-mannanase from T. virens UKM1 was successfully cloned and expressed as functionally active enzyme in P. pastoris.
class="label">•: Expressed recombinant mannanase (rMANTV) showed high optimum temperature at 70 °C.
class="label">•: It showed 70% of its activity at 80 °C and good stability along 20–65 °C.
class="label">•: rMANTV showed good thermotolerance with more than 60% of retaining activity at 60 °C up to one hour.
class="label">•: rMANTV has a half-life of 26 min at 70 °C.

NGLC 2004-2010.National Geological Library of China All Rights Reserved.
Add:29 Xueyuan Rd,Haidian District,Beijing,PRC. Mail Add: 8324 mailbox 100083
For exchange or info please contact us via email.