Characterization of a novel y-type HMW-GS with eight cysteine residues from Triticum monococcum ssp. monococcum

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Characterization of a novel y-type HMW-GS with eight cysteine residues from Triticum monococcum ssp. monococcum

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作者：Zenglin Li^a ; Hongyu Li^a ; Gang Chen^a ; Chunlan Kou^a ; Shunzong Ning^a ; Zhongwei Yuan^a ; Qi Jiang^b ; Youliang Zheng^a ; Dengcai Liu^a ; Lianquan Zhang^a ; ^{zhanglianquan1977@126.com" class="auth_mail" title="E-mail the corresponding author}
关键词：aa ; amino acid(s) ; bp ; base pair(s) ; BLAST ; basic local alignment search tool ; dNTP ; deoxyribonucleoside triphosphate ; HMW-GS ; high molecular weight glutenin subunit ; IPTG ; isopropyl 尾-螖-thiogalactopyranoside ; NCBI ; National Center for Biotechnology Information ; NJ ; Neighbor-joining ; ORF ; open reading frame ; PCR ; polymerase chain reaction ; SDS-PAGE ; sodium dodecyl sulfate polyacrylamide gel electrophoresis
刊名：Gene
出版年：2015
出版时间：15 November 2015
年：2015
卷：573
期：1
页码：110-114
全文大小：532 K

文摘

•: An active y-type HMW-GS allele 1Ay8.2 was identified from Triticum monococcum ssp. monococcum.
•: 1Ay8.2 contains eight cysteine residues.
•: 1Ay8.2 is expressed in a newly synthesized amphiploid (AAA^mA^mBB, 2n = 6x = 42).

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