High-throughput process development of an alternative platform for the production of virus-like particles in Escherichia coli

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High-throughput process development of an alternative platform for the production of virus-like particles in Escherichia coli

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作者：Christopher Ladd Effio^a ; ¹ ; ^{christopher.ladd@kit.edu" class="auth_mail" title="E-mail the corresponding author} ; Pascal Baumann^a ; ¹ ; ^{pascal.baumann@kit.edu" class="auth_mail" title="E-mail the corresponding author} ; Claudia Weigel^a ; Philipp Vormittag^a ; Anton Middelberg^b ; Jü ; rgen Hubbuch^a ; ^{juergen.hubbuch@kit.edu" class="auth_mail" title="E-mail the corresponding author} ;
关键词：Virus-like particles ; Escherichia coli ; Micro-scale cultivation ; BioLector ; High-throughput screening
刊名：Journal of Biotechnology
出版年：2016
出版时间：10 February 2016
年：2016
卷：219
期：Complete
页码：7-19
全文大小：3901 K

文摘

•: Establishing a HTS workflow for cell cultivation, lysis and analytics.
•: Optimizing the production of recombinant virus-like particles for chimeric vaccines.
•: Yielding 1.63 mg/mL VP1 with 43% TSP by codon optimization and design of experiments.
•: Developing a simple two-step downstream process for murine polyomavirus protein VP1.
•: Assembling VP1 into homogeneous VLPs with a size of 40–50 nm and a protein purity of 92%.

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