Non-cytotoxic organic-inorganic hybrid bioscaffolds: An efficient bedding for rapid growth of bone-like apatite and cell proliferation

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• 作者：?ukasz John ; Marta Ba?trukiewicz ; Piotr Sobota ; Renata Brykner ; ?ucja Cwynar-Zaj?c ; Piotr Dzi?giel
• 关键词：Hydroxyapatite ; Scaffold ; Bone tissue engineering ; Composite ; Biomineralization ; Cell proliferation
• 刊名：Materials Science and Engineering: C
• 出版年：2012
• 出版时间：1 October, 2012
• 年：2012
• 卷：32
• 期：7
• 页码：1849-1858
• 全文大小：2022 K

文摘

Synthesis and characterization of organic-inorganic macroporous hybrid scaffolds were investigated. The materials were prepared by combining 2-hydroxyethylmethacrylate (HEMA) and triethoxyvinylsilane (TEVS) chemically modified by Ca^2 + and PO₄^3 ? ions via sol-gel route. In this study we have constructed a sugar-based cracks-free three-dimensional (3D) network with interconnected porous architecture within the range of 150-300 ¦Ìm and rough topography. The obtained results revealed that both topography and composition of prepared materials allow rapid growth of the bone-like apatite (HAp) layer on their surface after soaking in biological medium. Preliminary studies have shown that hybrids covered by HAp are non-cytotoxic and allow cell proliferation that make them a promising scaffolds in the field of bone regenerative medicine. The materials were mainly characterized by powder X-ray diffraction analysis (PXRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) and transmission electron microscopy-energy-dispersive spectroscopy (TEM-EDS).