Cycloheximide-mediated superinduction of genes involves both native and foreign transcripts in rice (Oryza sativa L.)

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• 作者：Manu Agarwal ; Amanjot Singh ; Dheeraj Mittal ; Ch ; an Sahi ; Anil Grover
• 关键词：Cycloheximide ; Heat shock ; Promoter ; Rice (Oryza sativa L.) ; Superinduction ; Transcripts
• 刊名：Plant Physiology and Biochemistry
• 出版年：2011
• 出版时间：January 2011
• 年：2011
• 卷：49
• 期：1
• 页码：9-12
• 全文大小：363 K

文摘

Rice seedlings subjected to heat shock show rapid and transient induction of Oshsp17.4-CI, Oshsp17.9A-CI and OsClpB-cyt/hsp100 transcripts. When the seedlings were pre-treated with protein synthesis inhibitor cycloheximide, levels of the above transcripts during heat shock were more elevated than those seen with heat shock alone. Heat stress and cycloheximide co-treatment resulted in higher transcript accumulation in comparison to cycloheximide pre-treatment followed by heat stress. In transgenic plants raised with OsClpB-cyt/hsp100 promoter driving expression of the reporter gus gene, expression of the gus transcript was subjected to similar superinduction event as was seen with native OsClpB-cyt/hsp100 transcripts in untransformed plants. Yeast cells transformed with variably-sized rice ClpB-cyt/hsp100 promoter driving expression of the lacZ reporter transcript showed that specific sequences of the promoter region may be implicated in superinduction.