Discrimination of live and dead cells of Escherichia coli using propidium monoazide after sodium dodecyl sulfate treatment

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Discrimination of live and dead cells of Escherichia coli using propidium monoazide after sodium dodecyl sulfate treatment

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作者：Hajime Takahashi ; ^{hajime@kaiyodai.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Yun Gao ; Satoko Miya ; Takashi Kuda ; Bon Kimura
关键词：Propidium monoazide ; Escherichia coli ; Real-time PCR ; Sodium dodecyl sulfate
刊名：Food Control
出版年：2017
出版时间：January 2017
年：2017
卷：71
期：Complete
页码：79-82
全文大小：345 K

文摘

•: PMA-qPCR sometimes overestimates the number of live cells when mildly heat-stressed.
•: Single or double PMA treatment generated false-positive signals.
•: Combination of double PMA treatment and SDS eliminated false-positive signals.
•: This method could be used even when the viable cells occupied 0.01% of the total.

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