Differential modulation of the chaperone-like activity of HSP-1/2, a major protein of horse seminal plasma by anionic and cationic surfactants

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• 作者：C. Sudheer Kumar ; Musti J. Swamy ; ^{mjswamy@uohyd.ac.in} ; ^{mjswamy1@gmail.com} ;
• 关键词：ADH ; alcohol dehydrogenase ; Ala-16 ; alanine palmityl ester hydrochloride ; ANS ; 8-anilinonaphthalene-1-sulphonate ; CD ; circular dichroism ; CLA ; chaperone-like activity ; CTAB ; cetyl trimethylammonium bromide ; FnII ; fibronectin type-II ; HSP-1/2 ; horse seminal plasma proteins 1 and 2 ; SDS ; sodium dodecyl sulphate ; SDBS ; sodium dodecyl benzenesulphonate ; TBS ; 50  ; mM tris buffer containing 0.15  ; M NaCl ; 5  ; mM EDTA ; pH 7.4
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：524-531
• 全文大小：901 K
• 卷排序：96

文摘

Anionic and neutral surfactants inhibit thermal aggregation of ADH. Cationic surfactants induce aggregation of ADH even at room temperature. Anionic surfactants increase the CLA of HSP-1/2 whereas cationic surfactants decrease it. The above differences are explained based on electrostatic interactions.