Identification of Candida albicans exposed surface proteins in vivo by a rapid proteomic approach

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• 作者：Mar&#xed ; a Luisa Hern&#xe1 ; ez ; Pilar Xim&#xe9 ; nez-Emba ; n ; Montserrat Mart&#xed ; nez-Gomariz ; Mar&#xed ; a Dolores Guti&#xe9 ; rrez-Bl&#xe1 ; zquez ; C&#xe9 ; sar Nombela ; Concha Gil
• 关键词：Candida albicans cell wall ; LC-MALDI TOF/TOF ; Surface proteins digestion in vivo ; Yeast surfome
• 刊名：Journal of Proteomics
• 出版年：2010
• 出版时间：7 May 2010
• 年：2010
• 卷：73
• 期：7
• 页码：1404-1409
• 全文大小：272 K

文摘

We have set up a fast and easy methodology to identify cell-surface proteins in live yeasts. A non-gel proteomic approach was based on a short period of trypsin treatment followed by peptide separation and identification using nano-LC followed by off-line MS/MS. Candida albicans was used as a model organism and proteins involved in cell wall organization, cell rescue, defense, virulence, transport, protein fate and metabolism were identified. This strategy is a powerful tool to study host–pathogen interactions and to look for potential vaccine candidates and drug targets.