Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3

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• 作者：Arjan J. Groot ; Mohamed El Khattabi ; Norman Sachs ; Petra van der Groep ; Elsken van der Wall ; Paul J. van Diest ; Arnoud Sonnenberg ; C. Theo Verrips ; Marc Vooijs
• 关键词：Integrin ; Phage-display ; Surface marker ; Anti adhesive VHH ; VLA-3
• 刊名：Molecular Immunology
• 出版年：2009
• 出版时间：June 2009
• 年：2009
• 卷：46
• 期：10
• 页码：2022-2028
• 全文大小：1100 K

文摘

Therapeutic approaches aimed at targeting tumor surface markers using monoclonal antibodies provide a powerful strategy in cancer treatment. Here we report selection of single variable domains (VHH) of llama heavy chain antibodies, using a VHH-phage-display library. A reverse proteomic approach was used to identify the cognate proteins recognized by enriched VHH on HeLa cells. One of these VHH bound the integrin α3β1 (VLA-3) and was further characterized. Most interestingly, this VHH could inhibit VLA-3 mediated cell-matrix adhesion. Our approach provides a fast and efficient method to screen for novel cell surface markers on normal and tumor cells that may find diagnostic or therapeutic application in disease management or treatment.