Antimalarials have been determined in whole blood, plasma and urine.

Dried blood spot, protein precipitation and dilution are combined with liquid–liquid extraction, and solid-phase extraction.

The DBS method is safer than whole blood and good for field studies but can cause severe analyte losses.

Protein precipitation is good for lipophilic compounds but may cause degradation of artemisinin-based drugs.

SPE is frequently used for extraction of artemisinin and its derivates because it minimizes contact with organic solvents.