Functional characterization of two prokaryotic pentameric ligand-gated ion channel chimeras - role of the GLIC transmembrane domain in proton sensing

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• 作者：Camille M Hé ; nault ; John E Baenziger ; ^{John.Baenziger@uottawa.ca}
• 关键词：5HT3R ; serotonin receptor ; α-BTX ; α-bungarotoxin ; AChBP ; acetylcholine-binding protein ; ECD ; extracellular domain ; ELIC ; Erwinia ligand-gated ion channel ; GLIC ; Gloebacter ligand-gated ion channel ; GlyR ; glycine receptor ; nAChR ; nicotinic acetylcholine receptor ; pLGIC ; pentameric ligand-gated ion channel ; TMD ; transmembrane domain
• 刊名：Biochimica et Biophysica Acta (BBA) - Biomembranes
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1859
• 期：2
• 页码：218-227
• 全文大小：1487 K
• 卷排序：1859

文摘

GLIC-extracellular/ELIC transmembrane chimera exhibits impaired function ELIC-extracellular/GLIC transmembrane chimera (EG) gates open to an ELIC agonist EG gates open to protons at pH values below 4.5 Protons inhibit the EG agonist response 7 ≤ pH ≤ 4.5, but potentiate below pH 4.5 Thermal stability of chimeras is similar to that of the parent transmembrane domain