Expression analysis of ClpB/Hsp100 gene in faba bean (Vicia faba L.) plants in response to heat stress

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• 作者：Ritesh Kumar^a ; Amit Kumar Singh^a ; Dhruv Lavania^a ; Manzer H. Siddiqui^b ; Mohamed H. Al-Whaibi^b ; Anil Grover^a ; ^{anil.anilgrover@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ClpB ; Faba bean ; Heat stress ; Hsp100 ; Pollen ; Protein expression ; Thermotolerance ; Transcript induction
• 刊名：Saudi Journal of Biological Sciences
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：23
• 期：2
• 页码：243-247
• 全文大小：1072 K

文摘

Heat stress adversely affects the growth and yield of faba bean crop. Accumulation of ClpB/Hsp100 class of proteins is a critical parameter in induction of acquired heat stress tolerance in plants. Heat-induced expression of ClpB/Hsp100 genes has been noted in diverse plant species. Using primers complementary to soybean ClpB/Hsp100 gene, we analyzed the transcript expression profile of faba bean ClpB/Hsp100 gene in leaves of seedlings and flowering plants and in pollen grains. ClpB/Hsp100 protein accumulation profile was analyzed in leaves of faba bean seedlings using Arabidopsis thaliana cytoplasmic Hsp101 antibodies. The transcript and protein levels of faba bean ClpB/Hsp100 were significantly induced in response to heat stress.