A facile cascade signal amplification strategy using DNAzyme loop-mediated isothermal amplification for the ultrasensitive colorimetric detection of Salmonella

详细信息    查看全文

• 作者：Longjiao Zhu^aAuthor Vitae ; Yuancong Xu^aAuthor Vitae ; Nan Cheng^aAuthor Vitae ; Peiyan Xie^aAuthor Vitae ; Xiangli Shao^aAuthor Vitae ; Kunlun Huang^a ; ^bAuthor Vitae ; Yunbo Luo^a ; ^bAuthor Vitae ; Wentao Xu^a ; ^b ; ^{xuwentao@cau.edu.cn} ; ^{xuwentao1111@sina.com}Author Vitae
• 关键词：DNAzyme ; LAMP ; dLAMP ; Facile ; Ultrasensitive ; Salmonella
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：242
• 期：Complete
• 页码：880-888
• 全文大小：976 K
• 卷排序：242

文摘

Novel dLAMP sensor was developed by introducing DNAzymes into LAMP system. The method facilitates an ultrasensitive visual assay for even less 0.5 pg DNA. The principle to tailor DNAzymes compatible with LAMP was established. dLAMP sensor enhances the visual sensitivity of LAMP. Zero background was realized by introducing signal precursor.