Probing bulky ligand entry in engineered archaeal ferritins

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• 作者：Lorenzo Calisti^a ; Irene Benni^a ; Matilde Cardoso Trabuco^a ; Paola Baiocco^b ; Barbara Ruzicka^c ; Alberto Boffi^a ; ^d ; Elisabetta Falvo^d ; Francesco Malatesta^a ; Alessandra Bonamore^a ; ^{alessandra.bonamore@uniroma1.it} ; ^{alessandra.bonamore@gmail.com}
• 关键词：Self-assembly ; Binding kinetics ; Nano-scaffold ; Ferritin
• 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1861
• 期：2
• 页码：450-456
• 全文大小：703 K
• 卷排序：1861

文摘

A set of engineered archael ferritin mutants have been obtained. The mutants bear cysteine thiols in selected topological positions. Thiol reactivity has been probed using the bulky thiol reactive DTNB. Archaeal ferritins can bind DTNB outside but also inside the cavity. Protein matrix is not a significant barrier against bulky, negatively charged ligands.