Electrochemical detection of human ferritin based on gold nanorod reporter probe and cotton thread immunoassay device

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• 作者：Ting-Ting Song^a ; ¹ ; Wei Wang^b ; ¹ ; Li-Li Meng^a ; Yan Liu^a ; Xiao-Bo Jia^a ; Xun Mao^a ; ^{xunmao@nwu.edu.cn}
• 关键词：Cotton thread immunoassay device ; Gold nanorod reporter probe ; Point-of-care diagnosis ; Protein detection ; Electrochemical immunoassay
• 刊名：Chinese Chemical Letters
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：28
• 期：2
• 页码：226-230
• 全文大小：773 K
• 卷排序：28

文摘

In this study, a natural cotton thread immunoassay device combined with gold nanorod (GNR) reporter probe is developed for the rapid, sensitive and quantitative electrochemical determination of human ferritin, a lung cancer related biomarker. Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device. An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle (GNP) report probe. The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry (ASV) testing, which meet the requirement for clinical diagnosis.