Imaging flow cytometry in the assessment of leukocyte-platelet aggregates

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• 作者：Henry Hui ; Kathy A. Fuller ; Wendy N. Erber ; Matthew D. Linden ; ^{Matthew.linden@uwa.edu.au}
• 关键词：AF488 ; Alexafluor 488 ; APC ; allophycocyanin ; BP ; bandpass ; BSA ; bovine serum albumin ; BV421 ; Brilliant Violet 421 ; FCM ; flow cytometry ; IFC ; imaging flow cytometry ; ISX ; Amnis Imagestreamx ; LPA ; leukocyte-platelet aggregate ; MFI ; median fluorescence intensity ; MPA ; monocyte-platelet aggregate ; PSGL-1 ; P-Selectin glycoprotein ligand 1 ; RMS ; root mean square ; SD ; standard deviation ; TRAP ; thrombin receptor activating peptide (SFLLRN) ; WBC ; white blood cell (leukocyte)
• 刊名：Methods
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：112
• 期：Complete
• 页码：46-54
• 全文大小：2309 K
• 卷排序：112

文摘

Leukocyte-platelet aggregates are an important cellular interaction. Measurement with flow cytometry is confounded by untethered coincident cells. Imaging flow cytometry can resolve this issue to improve both enumeration and characterization.