Sodium polyanethol sulfonate (SPS) falsifies protein staining and quantification and how to solve this problem

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Sodium polyanethol sulfonate (SPS) falsifies protein staining and quantification and how to solve this problem

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作者：Marcel Prax¹ ; Shideh Vatani Shahmirzadi¹ ; Friedrich Gö ; tz ; ^{friedrich.goetz@uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author}
关键词：SPS ; sodium polyanethol sulfonate ; BCA ; bicinchoninic acid assay
刊名：Journal of Microbiological Methods
出版年：2015
出版时间：November 2015
年：2015
卷：118
期：Complete
页码：176-181
全文大小：664 K

文摘

•: Lysis inhibitor sodium polyanethol sulfonate (SPS) falsifies protein staining.
•: Several methods (precipitation, staining) were tested without success.
•: Only imidazole–zinc-staining and bicinchoninic acid assay overcome the problem.

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