The protective effect of total phenolics from Oenanthe Javanica on acute liver failure induced by D-galactosamine

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• 作者：Guo Ai^a ; ^b ; ^{guoair@163.com" class="auth_mail" title="E-mail the corresponding author} ; Zheng-Ming Huang^c ; ^{huangzhengming@cmea.org.cn" class="auth_mail" title="E-mail the corresponding author} ; Qing-Chuan Liu^c ; ^{frog207@163.com" class="auth_mail" title="E-mail the corresponding author} ; Yan-Quan Han^c ; ^{YHS786@139.com" class="auth_mail" title="E-mail the corresponding author} ; Xi Chen^d ; ^{ywc458@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：TPOJ ; Total Phenolics from Oenanthe Javanica ; TCM ; Traditional Chinese Medicine ; D-GalN ; D-Galactosamine ; iNOS ; Nitric Oxide Synthase ; COX-2 ; Cyclooxygenase-2 ; SOD ; Superoxide Dismutase ; GPx ; Glutathione Peroxidase ; CAT ; Catalase ; MDA ; Malondialdehyde ; GSH ; Glutathione ; NO ; Nitric Oxide ; PGE2 ; Prostaglandin E2 ; HBV ; Hepatitis B Virus ; AST ; Aspartate Aminotransferase ; ALT ; Alanine Aminotransferase ; ALP ; Alkaline Phosphatase ; TBIL ; Total Bilirubin ; GGT ; Gamma-Glutamyltransferase ; GAPDH ; Glyceralde
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：20 June 2016
• 年：2016
• 卷：186
• 期：Complete
• 页码：53-60
• 全文大小：864 K

文摘

Water dropwort [Oenanthe javanica (O. javanica)] is an aquatic perennial herb cultivated in East Asian countries. It has been popularly used in traditional Chinese medicine which is beneficial for the treatment of many diseases, including jaundice and various types of chronic and acute hepatitis. In the present study, we investigated the hepatoprotective effect of total phenolics from O. javanica (TPOJ) against D-galactosamine (D-GalN) induced liver injury in mice.

d="absSec_2">Material and methods

d="sp0055">The hepatoprotective activity of TPOJ (125, 250 and 500 mg/kg) was investigated on D-GalN (800 mg/kg)-induced liver damages in mice. Blood and liver were collected for biochemical and microscopic analysis. RT-PCR was used to determine the changes in hepatic nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Protein levels of iNOS, COX-2, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were determined by western blotting.

d="absSec_3">Results

d="sp0060">In the animal studies, TPOJ could improve the survival of acute liver failure model significantly and prevente the D-GalN-induced elevation of the serum enzymatic markers and nonenzymatic markers levels significantly. Meanwhile, TPOJ-treatment decreased the malondialdehyde (MDA) level and elevated the content of glutathione (GSH) in the liver as compared to those in the D-GalN group. Hepatic activities and protein expressions of antioxidative enzymes, including SOD, GPx, and CAT were enhanced dose dependently with TPOJ. At the same time, application of TPOJ effectively suppressed the D-GalN-induced proinflammatory mRNA and protein expression of iNOS and COX-2. Subsequently, the serum levels of proinflammatory mediators, nitric oxide (NO) and prostaglandin E₂ (PGE₂) were reduced. Additionally, histological analyses also showed that TPOJ reduced the extent of liver lesions induced by D-GalN.

d="absSec_4">Conclusion

d="sp0065">Our investigation demonstrated the hepatoprotective activity of TPOJ and revealed that TPOJ attributed its significance in the traditional use for treating liver diseases.