Ovary and embryo proteogenomic dataset revealing diversity of vitellogenins in the crustacean Gammarus fossarum

详细信息    查看全文

• 作者：Judith Trapp^a ; ^b ; Jean-Charles Gaillard^b ; Arnaud Chaumot^a ; Olivier Geffard^a ; Olivier Pible^b ; Jean Armengaud^b ; ^{jean.armengaud@cea.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Crustacean ; Embryogenesis ; Non-model organism ; Oogenesis ; Proteogenomics Reproduction ; Vitellogenins
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：8
• 期：Complete
• 页码：1259-1262
• 全文大小：625 K

文摘

Ovaries and embryos from sexually mature Gammarus fossarum were sampled at different stages of the reproductive cycle. The soluble proteome was extracted for five biological replicates and samples were subjected to trypsin digestion. The resulting peptides were analyzed by high resolution tandem mass spectrometry with a LTQ-Orbitrap XL instrument. The MS/MS spectra were assigned with a previously described RNAseq-derived G. fossarum database. The proteins highlighted by proteogenomics were monitored and their abundance kinetics over the different stages revealed a large panel of vitellogenins. Criteria were i) accumulation during oogenesis, ii) decrease during embryogenesis, iii) classified as female-specific, and iv) sequence similarity and phylogenetic analysis. The data accompanying the manuscript describing the database searches and comparative analysis (“High-throughput proteome dynamics for discovery of key proteins in sentinel species: unsuspected vitellogenins diversity in the crustacean Gammarus fossarum” by Trapp et al. [1]) have been deposited to the ProteomeXchange via the PRIDE repository with identifiers PRIDE: PXD001002.