Outbreak of fungemia caused by Candida parapsilosis in a neonatal intensive care unit: Molecular investigation through microsatellite analysis

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• 作者：Luciana da Silva Ruiz^a ; ^{lu_ruiz74@hotmail.com} ; Augusto Cezar Montelli^b ; Maria de F&aacute ; tima Sugizaki^b ; Eriques Gon&ccedil ; alves Da Silva^a ; Georgea Carla Matuura De Batista^a ; D&eacute ; bora Moreira^a ; Claudete Rodrigues Paula^a
• 关键词：Outbreak ; Candida parapsilosis ; Fungemia ; Neonates ; Microsatellite
• 刊名：Revista Iberoamericana de Micolog¨ªa
• 出版年：2013
• 出版时间：April-June, 2013
• 年：2013
• 卷：30
• 期：2
• 页码：112-115
• 全文大小：199 K

文摘

class=""h4"">Background

Opportunistic infections are an increasingly common problem in hospitals, and the yeast Candida parapsilosis has emerged as an important nosocomial pathogen, especially in neonatal intensive care units (NICUs) where it has been responsible for outbreak cases. Risk factors for C. parapsilosis infection in neonates include prematurity, very low birth weight, prolonged hospitalization, indwelling central venous catheters, hyperalimentation, intravenous fatty emulsions and broad spectrum antibiotic therapy. Molecular methods are widely used to elucidate these hospital outbreaks, establishing genetic variations among strains of yeast.

class=""h4"">Aims

The aim of this study was to detect an outbreak of C. parapsilosis in an NICU at the ¡°Hospital das Clinicas¡±, Faculty of Medicine of Botucatu, a tertiary hospital located in S?o Paulo, Brazil, using the molecular genotyping by the microsatellite markers analysis.

class=""h4"">Methods

A total of 11 cases of fungemia caused by C. parapsilosis were identified during a period of 43 days in the NICU. To confirm the outbreak all strains were molecularly typed using the technique of microsatellites.

class=""h4"">Results

Out of the 11 yeast samples studied, nine showed the same genotypic profile using the technique of microsatellites.

class=""h4"">Conclusions

Our study shows that the technique of microsatellites can be useful for these purposes. In conclusion, we detected the presence of an outbreak of C. parapsilosis in the NICU of the hospital analyzed, emphasizing the importance of using molecular tools, for the early detection of hospital outbreaks, and for the introduction of effective preventive measures, especially in NICUs.