Hydrophilic interaction liquid chromatography method development and validation for the assay of HEPES zwitterionic buffer

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• 作者：Xiaolong Xu ; Bert Gevaert ; Nathalie Bracke ; Han Yao ; Evelien Wynendaele ; Bart De Spiegeleer ; ^{Bart.DeSpiegeleer@UGent.be}
• 关键词：HILIC ; HEPES ; EPPS ; N-oxide degradant ; Zwitterionic
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：20 February 2017
• 年：2017
• 卷：135
• 期：Complete
• 页码：227-233
• 全文大小：892 K
• 卷排序：135

文摘

A method for the assay of HEPES was developed and validated. The influence of temperature, solvent strength, pH and ion concentration on the retention behavior was investigated. Four different retention models were applied to the retention data. A degradant forming under oxidative stressing condition was identified as N-oxide of HEPES. The final method was applied to the commercially available samples.