An optimized method to isolate glutenin macropolymer (GMP) gel is presented.
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Specific washing steps increase the polymer content.
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GMP gel contains protein, starch, water, and sodium dodecylsulphate.
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GMP gel is enriched in HMW glutenin subunits as compared to glutenin.
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Amount and composition of GMP gel are affected by wheat cultivar.
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