文摘
Babesia gibsoni is a tick-borne apicomplexan parasite of dogs that often causes fever and hemolytic anemia. Previous reports have shown that the apical membrane antigen 1 (BgAMA1), the 50 kDa surface antigen (BgP50), the secreted antigen 1 (BgSA1), and the thrombospondin-related adhesive protein (BgTRAP) are promising diagnostic antigens and vaccine candidates against B. gibsoni. In the present study, we investigated the genetic polymorphisms and natural selection of these four genes of B. gibsoni isolated from dogs in southwest Japan. The prediction B-cell epitopes showed high antigenic score in the insert and indel regions of BgSA1 and BgTRAP. Sequence analyses have revealed that BgAMA1 had the highest nucleotide diversity, followed by BgP50, BgSA1 and BgTRAP. Meanwhile, the Tajima's D value test suggested balancing selection for BgAMA1 and BgP50. However, BgSA1 and BgTRAP have purifying selection making them potential vaccine candidate and diagnostic antigens since they are highly conserved. Our findings provide the genetic basis for designing and testing the efficacy of diagnostic antigens as well as vaccine candidates against B. gibsoni.
