Volumetric absorptive microsampling (VAMS) as an alternative to conventional dried blood spots in the quantification of miltefosine in dried blood samples

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• 作者：A.E. Kip^a ; ^b ; ^a.kip@nki.nl ; K.C. Kiers^a ; H. Rosing^a ; J.H.M. Schellens^b ; ^c ; J.H. Beijnen^a ; ^b ; ^c ; T.P.C. Dorlo^b ; ^d
• 关键词：Miltefosine ; Volumetric absorptive microsampling (VAMS) ; Hematocrit ; Dried blood ; Bioanalysis ; LC&ndash ; MS/MS
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：20 February 2017
• 年：2017
• 卷：135
• 期：Complete
• 页码：160-166
• 全文大小：917 K
• 卷排序：135

文摘

Volumetric absorptive microsampling (VAMS) method validated for miltefosine quantification in human blood. This novel matrix sampling method eliminates variabilities due to blood spot volume and blood spot homogeneity. Developed fast and simple pre-treatment method with reproducible recovery. Assay performed well (bias within ±10.8%, CV% ≤ 11.9%) in wide validated range of 10–5000 ng/mL. Haematocrit impact on method accuracy reduced compared to conventional DBS sampling.