Cold treatment induced the release of Babesia bovis merozoites from infected RBCs.
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Viability of the merozoites was higher than those liberated by high-voltage pulses.
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The merozoites released by cold treatment invaded the RBCs effectively.
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The merozoites were useful in RBC invasion and invasion-inhibition assays.
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The cold treatment can be used to obtain viable and invasion-competent merozoites.
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