Detection of 1-O

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• 作者：Yutaka Abe ; Masayuki Tera ; Nobuhiro Sasaki ; Masachika Okamura ; Naoyuki Umemoto ; Masaki Momose ; Nobuo Kawahara ; Hiroyuki Kamakura ; Yukihiro Goda ; Kazuo Nagasawa ; Yoshihiro Ozeki
• 关键词：Acyltransferase ; Anthocyanin ; Carnation ; Malic acid ; Dianthus caryophyllus
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2008
• 出版时间：5 September 2008
• 年：2008
• 卷：373
• 期：4
• 页码：473-477
• 全文大小：433 K

文摘

Carnations have anthocyanins acylated with malate. Although anthocyanin acyltransferases have been reported in several plant species, anthocyanin malyltransferase (AMalT) activity in carnation has not been identified. Here, an acyl donor substance of AMalT, 1-O-β-d-malylglucose, was extracted and partially purified from the petals of carnation. This was synthesized chemically to analyze AMalT activity in a crude extract from carnation. Changes in the AMalT activity showed close correlation to the accumulation of pelargonidin 3-malylglucoside (Pel 3-malGlc) during the development of red petals of carnation, but neither AMalT activity nor Pel 3-malGlc accumulation was detectable in roots, stems and leaves.