Galactose functionalized injectable thermoresponsive microgels for sustained protein release

详细信息    查看全文

• 作者：Shao-Feng Lou ; Lei Wang ; Gareth R. Williams ; Huali Nie ; Jing Quan ; Limin Zhu
• 关键词：Poly(NIPAAm-co-VAGA) ; Injectable microgels ; Emulsion polymerization ; Glycopolymer ; Protein release
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：1 January, 2014
• 年：2014
• 卷：113
• 期：Complete
• 页码：368-374
• 全文大小：2304 K

文摘

Novel galactose functionalized thermoresponsive injectable microgels, poly(N-isopropylacrylamide-co-6-O-vinyladipoyl-d-galactose) P(NIPAAm-co-VAGA), have been fabricated using a combination of enzymatic transesterification and emulsion copolymerization. The microgels exhibit reversible temperature-responsive behavior, which can be tuned by varying the monomer feed ratio. The lower critical solution temperatures (LCSTs) of the materials are close to body temperature and can result in a rapid thermal gelation at 37 掳C. Field emission scanning electron microscopy showed the resultant microgels to have porous structures, and dynamic light scattering experiments indicated a dramatic reduction in particle size as solutions of the polymers are heated through the LCST. The polymers can be loaded with protein (bovine serum albumin; BSA), and in vitro studies showed that the BSA release kinetics depend upon the temperature and copolymer composition. Microgels based on P(NIPAAm-co-VAGA) could hence serve as candidates for site-specific sustained release drug delivery systems.