A full-length TMUV cDNA is generated using a PCR-based protocol.

Infectious viral particles are rescued from BHK-21 cells transfected with in vitro transcribed RNA.

Genomic characterization reveals that the rescued virus is genetically indistinguishable from the parental virus except genetic markers.

The rescued virus possesses similar growth properties in BHK-21 cells and virulence in mice with the parental virus.

Overall the technical platform will be useful for future investigations on the molecular mechanisms involved in replication and pathogenesis of TMUV.