Construction and characterization of a full-length infectious cDNA clone of foot-and-mouth disease virus strain O/JPN/2010 isolated in Japan in 2010

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• 作者：Tatsuya Nishi ; Hiroyuki Onozato¹ ; Seiichi Ohashi² ; Katsuhiko Fukai ; Manabu Yamada ; Kazuki Morioka ; Toru Kanno ; ³ ; ^{kannot@affrc.go.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Foot-and-mouth disease virus ; Full-length cDNA ; Pathogenicity ; O/JPN/2010 strain
• 刊名：Research in Veterinary Science
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：106
• 期：Complete
• 页码：165-169
• 全文大小：542 K

文摘

A full-length infectious cDNA clone of the genome of a foot-and-mouth disease virus isolated from the 2010 epidemic in Japan was constructed and designated pSVL-f02. Transfection of Cos-7 or IBRS-2 cells with this clone allowed the recovery of infectious virus. The recovered virus had the same in vitro characterization as the parental virus with regard to antigenicity in neutralization and indirect immunofluorescence tests, plaque size and one-step growth. Pigs were experimentally infected with the parental virus or the recombinant virus recovered from pSVL-f02 transfected cells. There were no significant differences in clinical signs or antibody responses between the two groups, and virus isolation and viral RNA detection from clinical samples were similar. Virus recovered from transfected cells therefore retained the in vitro characteristics and the in vivo pathogenicity of their parental strain. This cDNA clone should be a valuable tool to analyze determinants of pathogenicity and mechanisms of virus replication, and to develop genetically engineered vaccines against foot-and-mouth disease virus.