Characterization of stem cell-derived liver and intestinal organoids as a model system to study nuclear receptor biology

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• 作者：Ingrid T.G.W. Bijsmans^a ; Alexandra Milona^a ; Noortje Ijssennagger^a ; Ellen C.L. Willemsen^a ; José ; M. Ramos Pittol^a ; Johan W. Jonker^b ; Katja Lange^c ; Guido J.E.J. Hooiveld^c ; Saskia W.C. van Mil^a ; ^{S.W.C.vanMil@umcutrecht.nl}
• 关键词：ChIP ; chromatin immunoprecipitation ; DIC ; differential interference contrast ; DM ; differentiation medium ; DSG ; Di(N-succinimidyl) glutarate ; EM ; expansion medium ; Fxr ; farnesoid x receptor ; IPA ; Ingenuity Pathway Analysis ; Lxr ; Liver x receptor ; NR ; nuclear receptor ; OCA ; obeticholic acid ; Ppar ; Peroxisome proliferator-activated receptor ; RMA ; robust multiarray analysis ; Rosi ; Rosiglitazone
• 刊名：Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：1863
• 期：3
• 页码：687-700
• 全文大小：1081 K
• 卷排序：1863

文摘

NR mRNA expression and function is similar between organoids and respective tissues. Fxrα, Lxrα/β, Pparα/γ activation showed induction of multiple target genes. NR target genes were induced in WT, but not in NR −/− organoids. Organoids are easy to use and suitable to study NR biology and targetability. Trpm6, Slc26a3, Ang1, and Rnase4 are novel target genes of Fxr in the intestine.