Human neutrophil elastase inhibition studied by capillary electrophoresis with laser induced fluorescence detection and microscale thermophoresis

网站地图 | English | 公务邮箱

About the library

Background
History
Leadership
Organization

Opening Hours
Collections
Help Via Email

Electronic Information Resources

Human neutrophil elastase inhibition studied by capillary electrophoresis with laser induced fluorescence detection and microscale thermophoresis

详细信息查看全文

作者：Fayad Syntia ; Reine Nehmé ; ; ^{reine.nehme@univ-orleans.fr" class="auth_mail" title="E-mail the corresponding author} ; Bé ; rengè ; re Claude ; Philippe Morin
关键词：Enzyme ; Inhibition ; Laser induced fluorescence ; Microscale thermophoresis ; Transverse diffusion of laminar flow profiles
刊名：Journal of Chromatography A
出版年：2016
出版时间：29 January 2016
年：2016
卷：1431
期：Complete
页码：215-223
全文大小：1792 K

文摘

•: Development of online assay for human neutrophil elastase (HNE) inhibition by CZE-LIF.
•: DMSO induces adverse HNE agglomeration as shown by microscale thermophoresis (MST).
•: IC₅₀ of ursolic and oleanolic acid toward HNE accurately determined by CZE-LIF.
•: Comparable K_i constant determined by MST and CE which validated the developed assays.
•: MST and CE are complementary techniques for studying biomolecule interactions.

NGLC 2004-2010.National Geological Library of China All Rights Reserved.
Add:29 Xueyuan Rd,Haidian District,Beijing,PRC. Mail Add: 8324 mailbox 100083
For exchange or info please contact us via email.