Comparison of assays for the detection of West Nile virus antibodies in equine serum after natural infection or vaccination

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• 作者：Kinga Joó ; ^a ; ⁱ ; ^{joo.kinga@aotk.szie.hu} ; ^{jookinga@yahoo.com} ; Tamá ; s Bakonyi^b ; ^c ; ^{bakonyi.tamas@aotk.szie.hu} ; Ottó ; Szenci^a ; ^{szenci.otto@aotk.szie.hu} ; Sá ; ra Sá ; rdi^d ; ^{sardi.sara@gmail.com} ; Emőke Ferenczi^e ; ^{ferenczi.emoke@oek.antsz.hu} ; Mó ; nika Barna^b ; ^{monika.barna@aotk.szie.hu} ; Pé ; ter Malik^f ; ^{malik.peter@aotk.szie.hu} ; Zdenek Hubalek^g ; ^{zhubalek@brno.cas.cz} ; Orsolya Fehé ; r^a ; ^{ezustezust@gmail.com} ; Orsolya Kutasi^a ; ^h ; ^{kutasi.orsolya@aotk.szie.hu}
• 关键词：ELISA ; enzyme-linked immunosorbent assay ; HA ; haemagglutination ; HIT ; haemagglutination inhibition test ; MAC-ELISA ; IgM antibody capture ELISA ; NLC ; normal cell antigen ; PRNT ; plaque reduction neutralisation test ; TMB ; 3 ; 3&prime ; 5 ; 5&prime ; -tetramethylbenzidine ; WNRA ; West Nile recombinant antigen ; WNV ; West Nile Virus
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：183
• 期：Complete
• 页码：1-6
• 全文大小：559 K
• 卷排序：183

文摘

West Nile Virus is endemic in Hungary. HIT does not substitute ELISA or PRNT. HIT is useful to gain statistical information about tendencies of fixed population. Recent infection and vaccination cannot be differentiated by MAC ELISA. Protective antibodies could not always be measured 1 year after primary injections.