Setting up the cut-off level of a sensitive barcode lateral flow assay with magnetic nanoparticles

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• 作者：Vasily G. Panferov ; Irina V. Safenkova ; Anatoly V. Zherdev ; Boris B. Dzantiev ; ^{dzantiev@inbi.ras.ru}
• 关键词：Lateral flow assay ; Cut-off regulation ; Immunoassay ; Magnetic nanoparticles ; Potato virus x
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：164
• 期：Complete
• 页码：69-76
• 全文大小：796 K
• 卷排序：164

文摘

Barcode lateral flow immunoassay (LFIA) for potato virus X was developed. The LFIA cut-off levels were regulated up to 3 orders. Cut-off levels were varied by the changes of the antibodies’ affinity/concentration. The barcode LFIA allows distinguishing latent/serious plant infections. Magnetic concentration leads to a six-fold reduction of the LFIA detection limit.