Suppression subtractive hybridisation and real-time PCR for strain-specific quantification of the probiotic Bifidobacterium animalis BAN in broiler feed

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Suppression subtractive hybridisation and real-time PCR for strain-specific quantification of the probiotic Bifidobacterium animalis BAN in broiler feed

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作者：Silvia Fibi^a ; ^{silvia.fibi@biomin.net" class="auth_mail" title="E-mail the corresponding author} ; Viviana Klose^a ; Michaela Mohnl^a ; Barbara Weber^a ; Alexander G. Haslberger^b ; Verity Ann Sattler^b
关键词：SSH ; qPCR ; Probiotics ; Feed
刊名：Journal of Microbiological Methods
出版年：2016
出版时间：April 2016
年：2016
卷：123
期：Complete
页码：94-100
全文大小：525 K

文摘

•: A strain specific primer-pair was developed for Bifidobacterium animalis (DSM 16284).
•: Development of a real-time PCR assay to quantify B. animalis in feed
•: Quantification of inclusion levels in a range of four logs possible.
•: Confirmation of the applicability in a spiking trial

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