Consequences of acute oxidative stress in Leishmania amazonensis: From telomere shortening to the selection of the fittest parasites

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• 作者：Marcelo Santos da Silva^a ; ^b ; ^c ; Marcela Segatto^a ; Raphael Souza Pavani^c ; Fernanda Gutierrez-Rodrigues^d ; Vanderson da Silva Bispo^e ; Marisa Helena Gennari de Medeiros^e ; Rodrigo Tocantins Calado^d ; Maria Carolina Elias^c ; Maria Isabel Nogueira Cano^a ; ^{micano@ibb.unesp.br}
• 关键词：Leishmania amazonensis ; Oxidative stress ; Telomere shortening ; LaRPA-1 ; DNA repair
• 刊名：Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：1864
• 期：1
• 页码：138-150
• 全文大小：1864 K
• 卷排序：1864

文摘

Acute oxidative stress increased intracellular ROS, caused DNA oxidation and damage. Oxidation induced LaRPA-1 dissociation from the 3′ G-overhang and telomere shortening. LaRPA-1 act as a SSB protecting the C-rich single-strand telomeric gaps generated upon oxidation. Oxidation caused death in part of the population and cell-cycle arrest in the survivors. Survivors continued replicating DNA, were more resistant to H2O2 and recovered telomere length.