Fluorescent sensor assay for β-lactamase in milk based on a combination of aptamer and graphene oxide

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• 作者：Jing Qin^a ; ^{jingqin1002@163.com} ; Xiping Cui^a ; ^{cuixiping1989@163.com} ; Panpan Wu^a ; ^{wyuwpp@126.com} ; Zhengyun Jiang^a ; ^{jkk928@163.com} ; Yingshan Chen^a ; ^{chenysgdut@163.com} ; Ruili Yang^b ; ^{rlyang77@scau.edu.cn} ; Qingqing Hu^a ; ^{18320408023@163.com} ; Yuanming Sun^b ; ^{ymsun@scau.edu.cn} ; Suqing Zhao^a ; ^{sqzhao@gdut.edu.cn}
• 关键词：Fluorescence sensor assay ; β-lactamase ; Aptamer ; Graphene oxide
• 刊名：Food Control
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：73
• 期：part_PB
• 页码：726-733
• 全文大小：1316 K
• 卷排序：73

文摘

Graphene oxide (GO) is proposed to be able to quench the fluorescence signal. β-lactamase aptamer was used to replace the traditional antibody. Based on aptamer assembled onto GO, a new, sensitive and direct determination of β-lactamase was established. The established method was verified by ELISA.