One facile fluorescence strategy for sensitive detection of endonuclease activity using DNA-templated copper nanoclusters as signal indicators

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One facile fluorescence strategy for sensitive detection of endonuclease activity using DNA-templated copper nanoclusters as signal indicators

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作者：Hengzhi ZhaoAuthor Vitae ; Jingjing DongAuthor Vitae ; Fulin ZhouAuthor Vitae ; Baoxin Li ; ^{libaoxin@snnu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; ^{libaoxin@snnu.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
关键词：Endonuclease activity ; Copper nanoclusters ; Fluorescence assay ; Label-free
刊名：Sensors & Actuators: B. Chemical
出版年：2017
出版时间：January 2017
年：2017
卷：238
期：Complete
页码：828-833
全文大小：1141 K

文摘

•: A simple and facile method is proposed for sensitive detection of endonuclease activity.
•: DNA-templated CuNCs can be used as signal indicators for fluorescence assay of endonuclease activity.
•: The method offers a convenient “mix-and-detect” homogeneous assay format without separation, precipitation and washing.
•: The whole assay process can be completed in a single tube.

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