Mild solubilization of aggregated VAS-TRAIL was achieved by a triple-denature solution consisting of urea, l-arginine, and alkaline PH.
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VAS-TRAIL solubilized by the triple-denature solution resulted in an efficient recovery of soluble protein over 50%.
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Refolded VAS-TRAIL fusion protein exhibited apoptotic activities toward tumor and endothelial cells.
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