Role of the spinal Na⁺/H⁺ exchanger in formalin-induced nociception

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• 作者：Gabriela Castañ ; eda-Corral^a ; H&#xe9 ; ctor I. Rocha-Gonz&#xe1 ; lez^b ; Beatriz God&#xed ; nez-Chaparro^a ; Juan Miguel Jim&#xe9 ; nez-Andrade^c ; Vinicio Granados-Soto^a ; ^{vgranados@prodigy.net.mx"" rel=""nofollow}
• 关键词：NHE1 ; NHE inhibitors ; Zoniporide ; NHE1 like-immunoreactivity ; Spinal processing
• 刊名：Neuroscience Letters
• 出版年：2011
• 出版时间：21 August 2011
• 年：2011
• 卷：501
• 期：1
• 页码：4-9
• 全文大小：1369 K

文摘

This study assessed the role of the Na⁺/H⁺ exchanger (NHE) in the formalin-induced nociception as well as the expression of the NHE isoform 1 (NHE1) in the rat spinal cord by using immunohistochemistry. Rats received a 50 μl injection of diluted formalin (0.5 % ). Nociceptive behavior was quantified as the number of flinches of the injected paw. Intrathecal administration of the partially selective NHE1 inhibitors DMA, EIPA (0.3–30 μM/rat) and the selective NHE1 inhibitor zoniporide (0.03–3 μM/rat) significantly increased formalin-induced flinching behavior in a dose-dependent manner during both phases of the test. Immunohistochemical analysis of the rat lumbar spinal cord showed that NHE1 was mainly expressed in the lamina I of the dorsal horn of the spinal cord. Double immunofluorescence staining showed co-localization of NHE1 with the peptide-rich sensory nerve fiber markers, substance P and calcitonin gene-related peptide, but not with markers of neuronal cell bodies (NeuN), microglia (OX-42) or astroglia (GFAP). Collectively, these pharmacological and anatomical results suggest that spinal NHE1 plays a role in formalin-induced nociception acting as a protective protein extruding H⁺.